Other Names Dopamine- and cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B; PPR1B
All Sites Human: Thr34; Mouse: Thr34; Rat: Thr34
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 34 (R-P-TP-P-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.
ReferencesOhta S., FEBS Lett. 242-31-35(1988).
Kemmer D., BMC Genomics 7:48-48(2006).
Martin J., Nature 432-988-994(2004).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells