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DARPP-32 (Phospho-Thr34) Antibody
Datasheet   

  • Catalog Number:

    E11-7056A

  • Amount:

    100μg

  • Swiss-Prot No.:

    Q9UD71

  • Other Names:

    Dopamine- and cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B; PPR1B

  • All Sites:

    Human: Thr34; Mouse: Thr34; Rat: Thr34

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 34 (R-P-TP-P-A).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.

  • Reactivity:

    Human; Mouse; Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

  • References:

    Ohta S., FEBS Lett. 242-31-35(1988).
    Kemmer D., BMC Genomics 7:48-48(2006).
    Martin J., Nature 432-988-994(2004).

  • P-peptide - +

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using DARPP-32 (Phospho-Thr34) antibody.
    Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using DARPP-32 (Phospho-Thr34) antibody.

  • P-peptide - +
    Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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DARPP-32 (Phospho-Thr34) Antibody | EnoGene Biotech Co. Ltd