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CREB (Phospho-Ser133) Antibody
Datasheet
Catalog Number:
E11-7053AAmount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 133 (R-P-SP-Y-R) (CILSRRPSYRKILN).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
CREB (phospho-Ser133) antibody detects endogenous levels of CREB only when phosphorylated at serine 133.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various DilutionSwiss-Prot No.:
P16220References:
Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al. (1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
- P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (phospho-Ser133) antibody. 
- Western blot analysis of extracts from HeLa cells treated with UV, using CREB (phospho-Ser133) antibody.
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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