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Cortactin (Phospho-Tyr466) Antibody
Datasheet
Catalog Number:
E11-7050AAmount:
100μg/100μlSwiss-Prot No.:
Q14247Altername:
Amplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactinAll Sites:
Human: Tyr470; Mouse: Tyr466; Rat: Tyr428Form of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Cortactin (phospho-Tyr466) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.Reactivity:
Human; Mouse; RatApplications:
WB: 1:500~1:1000 IHC:1:50~1:100
ELISA: 1:20000References:
Julie A. Head, Mol. Biol. Cell, Aug 2003; 14: 3216.
Cherry Luo, J. Biol. Chem., Oct 2006; 281: 30081 - 30093.
Jianwei Zhu, J. Biol. Chem., Apr 2007; 10.1074.
David Hattan, J. Biol. Chem., Oct 2002; 277: 38596 - 38606.
- P-peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin (phospho-Tyr466) antibody. 
- Western blot analysis of extracts from cos-7cells using Cortactin (phospho-Tyr466) antibody (Line 1 and 2).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from cos-7cells using Cortactin (phospho-Tyr466) antibody (Line 1 and 2).
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