All Names
AlternameAmplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactin
All SitesHuman: Tyr470; Mouse: Tyr466; Rat: Tyr428
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCortactin (phospho-Tyr466) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.
ReferencesJulie A. Head, Mol. Biol. Cell, Aug 2003; 14: 3216.
Cherry Luo, J. Biol. Chem., Oct 2006; 281: 30081 - 30093.
Jianwei Zhu, J. Biol. Chem., Apr 2007; 10.1074.
David Hattan, J. Biol. Chem., Oct 2002; 277: 38596 - 38606.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells