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Primary Antibodies

Cortactin (Phospho-Tyr466) Antibody

E11-7050ACite ID: EN0025411DatasheetCOAMSDS
Human; Mouse; Rat
WB: 1:500~1:1000 IHC:1:50~1:100
ELISA: 1:20000
Q14247
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameAmplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactin
All SitesHuman: Tyr470; Mouse: Tyr466; Rat: Tyr428

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCortactin (phospho-Tyr466) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.
ReferencesJulie A. Head, Mol. Biol. Cell, Aug 2003; 14: 3216.
Cherry Luo, J. Biol. Chem., Oct 2006; 281: 30081 - 30093.
Jianwei Zhu, J. Biol. Chem., Apr 2007; 10.1074.
David Hattan, J. Biol. Chem., Oct 2002; 277: 38596 - 38606.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Cortactin Western blot analysis using Cortactin (Phospho-Tyr466) Antibody. Line 1: Blocking peptide; Line 2: The extracts from COS7 cells ; Line 3: The extracts from JK cells. Use Only
Cortactin Western blot analysis using Cortactin (Phospho-Tyr466) Antibody. Line 1: Blocking peptide; Line 2: The extracts from COS7 cells ; Line 3: The extracts from JK cells. Use Only
Western blot analysis of extracts from cos-7cells using Cortactin (phospho-Tyr466) antibody (Line 1 and 2).
Western blot analysis of extracts from cos-7cells using Cortactin (phospho-Tyr466) antibody (Line 1 and 2).

Conjugated Antibody

This product is also available conjugated. Format: E11-7050A-Conjugate. Price: $485 / Size: 100μl.

E11-7050A-FITC$485 / 100μlE11-7050A-Biotin$485 / 100μlE11-7050A-HRP$485 / 100μlE11-7050A-AP$485 / 100μlE11-7050A-AF350$485 / 100μlE11-7050A-AF405M$485 / 100μlE11-7050A-AF488$485 / 100μlE11-7050A-AF514$485 / 100μlE11-7050A-AF532$485 / 100μlE11-7050A-AF555$485 / 100μlE11-7050A-AF568$485 / 100μlE11-7050A-AF405S$485 / 100μlE11-7050A-AF405L$485 / 100μlE11-7050A-AF546$485 / 100μlE11-7050A-AF594$485 / 100μlE11-7050A-AF610$485 / 100μlE11-7050A-AF635$485 / 100μlE11-7050A-AF647$485 / 100μlE11-7050A-AF680$485 / 100μlE11-7050A-AF700$485 / 100μlE11-7050A-AF750$485 / 100μlE11-7050A-AF790$485 / 100μlE11-7050A-APC$485 / 100μlE11-7050A-PE$485 / 100μlE11-7050A-Cy3$485 / 100μlE11-7050A-Cy5$485 / 100μlE11-7050A-Cy5.5$485 / 100μlE11-7050A-Cy7$485 / 100μlE11-7050A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.