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Primary Antibodies

Cortactin (Phospho-Tyr421) Antibody

E11-7049ACite ID: EN0025409DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:20000
Q14247
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameAmplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactin
All SitesHuman: Tyr421; Mouse: Tyr421; Rat: Tyr383

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCortactin (phospho-Tyr421) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 421.
ReferencesDu, Y. et al. (1998) Mol. Cell. Biol. 18, 5838-5851.
Naisbitt, S. et al. (1999) Neuron 23, 569-582.
Rodrigo, J. P. et al. (2000) Clin. Cancer Res. 6, 3177-3182.
Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-6434.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from Hela cells untreated or treated with H 2 O 2 , using Cortactin (Ab-421) antibody (Line 1 and 2) and Cortactin (phospho-Tyr421) antibody (Line 3 and 4).
Western blot analysis of extracts from Hela cells untreated or treated with H 2 O 2 , using Cortactin (Ab-421) antibody (Line 1 and 2) and Cortactin (phospho-Tyr421) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7049A-Conjugate. Price: $485 / Size: 100μl.

E11-7049A-FITC$485 / 100μlE11-7049A-Biotin$485 / 100μlE11-7049A-HRP$485 / 100μlE11-7049A-AP$485 / 100μlE11-7049A-AF350$485 / 100μlE11-7049A-AF405M$485 / 100μlE11-7049A-AF488$485 / 100μlE11-7049A-AF514$485 / 100μlE11-7049A-AF532$485 / 100μlE11-7049A-AF555$485 / 100μlE11-7049A-AF568$485 / 100μlE11-7049A-AF405S$485 / 100μlE11-7049A-AF405L$485 / 100μlE11-7049A-AF546$485 / 100μlE11-7049A-AF594$485 / 100μlE11-7049A-AF610$485 / 100μlE11-7049A-AF635$485 / 100μlE11-7049A-AF647$485 / 100μlE11-7049A-AF680$485 / 100μlE11-7049A-AF700$485 / 100μlE11-7049A-AF750$485 / 100μlE11-7049A-AF790$485 / 100μlE11-7049A-APC$485 / 100μlE11-7049A-PE$485 / 100μlE11-7049A-Cy3$485 / 100μlE11-7049A-Cy5$485 / 100μlE11-7049A-Cy5.5$485 / 100μlE11-7049A-Cy7$485 / 100μlE11-7049A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.