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Cortactin (Phospho-Tyr421) Antibody
Datasheet   

  • Catalog Number:

    E11-7049A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    Q14247

  • Altername:

    Amplaxin, CTTN, EMS1, Oncogene EMS1, SRC8, Src substrate cortactin

  • All Sites:

    Human: Tyr421; Mouse: Tyr421; Rat: Tyr383

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    Cortactin (phospho-Tyr421) antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 421.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:20000

  • References:

    Du, Y. et al. (1998) Mol. Cell. Biol. 18, 5838-5851.
    Naisbitt, S. et al. (1999) Neuron 23, 569-582.
    Rodrigo, J. P. et al. (2000) Clin. Cancer Res. 6, 3177-3182.
    Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-6434.

  • Western blot analysis of extracts from Hela cells untreated or treated with H2O2, using Cortactin (Ab-421) antibody (Line 1 and 2) and Cortactin (phospho-Tyr421) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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Cortactin (Phospho-Tyr421) Antibody | EnoGene Biotech Co. Ltd