All Names
AlternameCx43; Gap junction 43 kDa heart protein
All SitesHuman: Ser368; Mouse: Ser368; Rat: Ser368
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Connexin43 around the phosphorylation site of serine 368 (R-A-SP-S-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityConnexin43 (Phospho-Ser368) antibody detects endogenous levels of Connexin43 only when phosphorylated at serine 368.
ReferencesXiaoyong Bao, et al. (2004) Am J Physiol Cell Physiol; 286: C647-C654.
K Boengler, et al. (2006) Heart; 92: 1724-1727.
Satoshi Matsushita, et al. (2006) J. Histochem. Cytochem; 54: 343-353.
Holger K. Eltzschig, et al. (2006) Circ. Res; 99: 1100-1108.