All Names
AlternameCHEK1, Chk1, kinase Chk1
All SitesHuman: Ser280; Mouse: Ser280; Rat: Ser280
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 280 (V-T-SP-G-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityChk1 (phospho-Ser280) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 280.
ReferencesPuc et al. (2005) J Cancer Cell, 7, 2.
Yuchi Hirose, et al. (2005) Cancer Research 65, 4861-4869, June 1.
Vincent O'Brien, et al. (2006) Carcinogenesis 27(4):682-692.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells