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Primary Antibodies

Chk1 (Phospho-Ser280) Antibody

E11-7040ACite ID: EN0025391DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:20000
O14757
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameCHEK1, Chk1, kinase Chk1
All SitesHuman: Ser280; Mouse: Ser280; Rat: Ser280

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 280 (V-T-SP-G-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityChk1 (phospho-Ser280) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 280.
ReferencesPuc et al. (2005) J Cancer Cell, 7, 2.
Yuchi Hirose, et al. (2005) Cancer Research 65, 4861-4869, June 1.
Vincent O'Brien, et al. (2006) Carcinogenesis 27(4):682-692.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HT29 cells untreated or treated UV, using Chk1 (phospho-Ser280) antibody (Line 1 and 2).
Western blot analysis of extracts from HT29 cells untreated or treated UV, using Chk1 (phospho-Ser280) antibody (Line 1 and 2).

Conjugated Antibody

This product is also available conjugated. Format: E11-7040A-Conjugate. Price: $485 / Size: 100μl.

E11-7040A-FITC$485 / 100μlE11-7040A-Biotin$485 / 100μlE11-7040A-HRP$485 / 100μlE11-7040A-AP$485 / 100μlE11-7040A-AF350$485 / 100μlE11-7040A-AF405M$485 / 100μlE11-7040A-AF488$485 / 100μlE11-7040A-AF514$485 / 100μlE11-7040A-AF532$485 / 100μlE11-7040A-AF555$485 / 100μlE11-7040A-AF568$485 / 100μlE11-7040A-AF405S$485 / 100μlE11-7040A-AF405L$485 / 100μlE11-7040A-AF546$485 / 100μlE11-7040A-AF594$485 / 100μlE11-7040A-AF610$485 / 100μlE11-7040A-AF635$485 / 100μlE11-7040A-AF647$485 / 100μlE11-7040A-AF680$485 / 100μlE11-7040A-AF700$485 / 100μlE11-7040A-AF750$485 / 100μlE11-7040A-AF790$485 / 100μlE11-7040A-APC$485 / 100μlE11-7040A-PE$485 / 100μlE11-7040A-Cy3$485 / 100μlE11-7040A-Cy5$485 / 100μlE11-7040A-Cy5.5$485 / 100μlE11-7040A-Cy7$485 / 100μlE11-7040A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.