Other Names CDC25M1; dual specificity phosphatase Cdc25C; EC 3.1.3.48; M-phase inducer phosphatase 3; MPI1B; MPIP3
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CDC25C (Phospho-Ser216) antibody detects endogenous levels of CDC25C only when phosphorylated at serine 216.
ReferencesSadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990).
Bureik M., Int. J. Oncol. 17:1251-1258(2000).
Livingston R.J., Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.