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Primary Antibodies

CDC2 (Phospho-Thr161) Antibody

E11-7035ACite ID: EN0025379DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
P06493
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (T-Y-TP-H-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityCDC2(Phospho-Thr161) Antibody detects endogenous levels of CDC2 only when phosphorylated at Threonine 161.
ReferencesUkomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.
Liu Y, et al.(2004)J Biol Chem; 279(6): 4507-14.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using CDC2(Phospho-Thr161) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using CDC2(Phospho-Thr161) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Western blot analysis using CDC2 (Phospho-Thr161) Antibody: Line1: The extracts from COLO cells using preincubated with synthesized phosphopeptide; Line2: The extracts from COLO cells. Line3: The extracts from K562 cells.
Western blot analysis using CDC2 (Phospho-Thr161) Antibody: Line1: The extracts from COLO cells using preincubated with synthesized phosphopeptide; Line2: The extracts from COLO cells. Line3: The extracts from K562 cells.

Conjugated Antibody

This product is also available conjugated. Format: E11-7035A-Conjugate. Price: $485 / Size: 100μl.

E11-7035A-FITC$485 / 100μlE11-7035A-Biotin$485 / 100μlE11-7035A-HRP$485 / 100μlE11-7035A-AP$485 / 100μlE11-7035A-AF350$485 / 100μlE11-7035A-AF405M$485 / 100μlE11-7035A-AF488$485 / 100μlE11-7035A-AF514$485 / 100μlE11-7035A-AF532$485 / 100μlE11-7035A-AF555$485 / 100μlE11-7035A-AF568$485 / 100μlE11-7035A-AF405S$485 / 100μlE11-7035A-AF405L$485 / 100μlE11-7035A-AF546$485 / 100μlE11-7035A-AF594$485 / 100μlE11-7035A-AF610$485 / 100μlE11-7035A-AF635$485 / 100μlE11-7035A-AF647$485 / 100μlE11-7035A-AF680$485 / 100μlE11-7035A-AF700$485 / 100μlE11-7035A-AF750$485 / 100μlE11-7035A-AF790$485 / 100μlE11-7035A-APC$485 / 100μlE11-7035A-PE$485 / 100μlE11-7035A-Cy3$485 / 100μlE11-7035A-Cy5$485 / 100μlE11-7035A-Cy5.5$485 / 100μlE11-7035A-Cy7$485 / 100μlE11-7035A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.