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CDC2 (Phospho-Thr161) Antibody
Datasheet   

  • Catalog Number:

    E11-7035A

  • Amount:

    100μg/100μl

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (T-Y-TP-H-E).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    CDC2(Phospho-Thr161) Antibody detects endogenous levels of CDC2 only when phosphorylated at Threonine 161.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
    ELISA: 1:5000

  • Swiss-Prot No.:

    P06493

  • References:

    Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.
    Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.
    Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.
    Liu Y, et al.(2004)J Biol Chem; 279(6): 4507-14.

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma, using CDC2(Phospho-Thr161) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

  • Western blot analysis using CDC2 (Phospho-Thr161) Antibody: Line1: The extracts from COLO cells using preincubated with synthesized phosphopeptide; Line2: The extracts from COLO cells. Line3: The extracts from K562 cells.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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CDC2 (Phospho-Thr161) Antibody | EnoGene Biotech Co. Ltd