All Names
AlternameBRCA1, Breast cancer type 1 susceptibility protein
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityBRCA1 (phospho-Ser1423) antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1423.
ReferencesKim ST, et al. (2002) Genes Dev; 16(5): 560-570.
Arlt MF, et al. (2004) Mol Cell Biol; 24(15): 6701-6709.
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718.
Greenberg RA, et al. (2006) Genes Dev; 20(1): 34-46.
Tibbetts RS, et al. (2000) Genes Dev; 14(23): 2989-3002.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells