Other Names Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; BCL2
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity BCL-2 (Phospho-Ser70) antibody detects endogenous levels of BCL-2 only when phosphorylated at serine 70.
ReferencesTsujimoto Y., Proc. Natl. Acad. Sci. U.S.A. 83:5214-5218(1986).
Eguchi Y., Nucleic Acids Res. 20:4187-4192(1992).
Cleary M.L., Cell 47:19-28(1986).