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Primary Antibodies

ATF-2 (Phospho-Thr71 or 53) Antibody

E11-7015ACite ID: EN0025341DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000 IP: Various Dilution
P15336
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of Threonine 71 or 53.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityATF-2 (Phospho-Thr71 or 53) Antibody detects endogenous levels of ATF-2 only when phosphorylated at Threonine 71 or 53.
ReferencesSevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.
Waetzig G H, et al. (2002) J Immunol. 168(10): 5342-5351.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Price225$
Immunohistochemical analysis of paraffin- breast carcinoma. Left: Using ATF-2 (Phospho-Thr71 or 53) Antibody; Line2: The same preincubated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin- breast carcinoma. Left: Using ATF-2 (Phospho-Thr71 or 53) Antibody; Line2: The same preincubated with synthesized phosphopeptide.
embedded Western blot analysis of extracts, using ATF-2 (Phospho-Thr71 or 53) Antibody. Line1: The antibody extracts from HeLa cells untreated; Line2: The extracts from HeLa cells treated with UV.
embedded Western blot analysis of extracts, using ATF-2 (Phospho-Thr71 or 53) Antibody. Line1: The antibody extracts from HeLa cells untreated; Line2: The extracts from HeLa cells treated with UV.

Conjugated Antibody

This product is also available conjugated. Format: E11-7015A-Conjugate. Price: $485 / Size: 100μl.

E11-7015A-FITC$485 / 100μlE11-7015A-Biotin$485 / 100μlE11-7015A-HRP$485 / 100μlE11-7015A-AP$485 / 100μlE11-7015A-AF350$485 / 100μlE11-7015A-AF405M$485 / 100μlE11-7015A-AF488$485 / 100μlE11-7015A-AF514$485 / 100μlE11-7015A-AF532$485 / 100μlE11-7015A-AF555$485 / 100μlE11-7015A-AF568$485 / 100μlE11-7015A-AF405S$485 / 100μlE11-7015A-AF405L$485 / 100μlE11-7015A-AF546$485 / 100μlE11-7015A-AF594$485 / 100μlE11-7015A-AF610$485 / 100μlE11-7015A-AF635$485 / 100μlE11-7015A-AF647$485 / 100μlE11-7015A-AF680$485 / 100μlE11-7015A-AF700$485 / 100μlE11-7015A-AF750$485 / 100μlE11-7015A-AF790$485 / 100μlE11-7015A-APC$485 / 100μlE11-7015A-PE$485 / 100μlE11-7015A-Cy3$485 / 100μlE11-7015A-Cy5$485 / 100μlE11-7015A-Cy5.5$485 / 100μlE11-7015A-Cy7$485 / 100μlE11-7015A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.