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Primary Antibodies

ATF2 (Phospho-Thr69 or 51) Antibody

E11-7014ACite ID: EN0025339DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:3000 IHC: 1:50~1:100
ELISA: 1:20000 IP: Various Dilution
P15336
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of Threonine 69 or 51.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityATF-2(Phospho-Thr69 or 51) Antibody detects endogenous levels of ATF-2 only when phosphorylated at Threonine 69 or 51.
ReferencesSevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.
Alsayed Y, et al. (2001) J Biol Chem. 276(6): 4012-4019.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from 3T3 cells, using ATF-2 (Phospho-Thr69 or 51) Antibody.
Western blot analysis of extracts from 3T3 cells, using ATF-2 (Phospho-Thr69 or 51) Antibody.
Western blot analysis of extracts from LOVO cells. Line1: Using ATF-2 (Phospho-Thr69 or 51) Antibody; Line2: Using the same antibody preincubated with synthesized peptide.
Western blot analysis of extracts from LOVO cells. Line1: Using ATF-2 (Phospho-Thr69 or 51) Antibody; Line2: Using the same antibody preincubated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF-2 (Phospho-Thr69 or 51) Antibody Antibody; Right: The same antibody preincubated with synthesized phospho-peptide.
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ATF-2 (Phospho-Thr69 or 51) Antibody Antibody; Right: The same antibody preincubated with synthesized phospho-peptide.

Conjugated Antibody

This product is also available conjugated. Format: E11-7014A-Conjugate. Price: $485 / Size: 100μl.

E11-7014A-FITC$485 / 100μlE11-7014A-Biotin$485 / 100μlE11-7014A-HRP$485 / 100μlE11-7014A-AP$485 / 100μlE11-7014A-AF350$485 / 100μlE11-7014A-AF405M$485 / 100μlE11-7014A-AF488$485 / 100μlE11-7014A-AF514$485 / 100μlE11-7014A-AF532$485 / 100μlE11-7014A-AF555$485 / 100μlE11-7014A-AF568$485 / 100μlE11-7014A-AF405S$485 / 100μlE11-7014A-AF405L$485 / 100μlE11-7014A-AF546$485 / 100μlE11-7014A-AF594$485 / 100μlE11-7014A-AF610$485 / 100μlE11-7014A-AF635$485 / 100μlE11-7014A-AF647$485 / 100μlE11-7014A-AF680$485 / 100μlE11-7014A-AF700$485 / 100μlE11-7014A-AF750$485 / 100μlE11-7014A-AF790$485 / 100μlE11-7014A-APC$485 / 100μlE11-7014A-PE$485 / 100μlE11-7014A-Cy3$485 / 100μlE11-7014A-Cy5$485 / 100μlE11-7014A-Cy5.5$485 / 100μlE11-7014A-Cy7$485 / 100μlE11-7014A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.