Other Names Apoptosis signal- regulating kinase 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen-activated protein kinase kinase kinase 5
All Sites Human: Ser966; Mouse: Ser973
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 966 (S-I-SP-L-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ASK1 (Phospho-Ser966) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 966.
ReferencesWang X.S., J. Biol. Chem. 271:31607-31611(1996).
Ichijo H., Science 275:90-94(1997).
Mungall A.J., Nature 425:805-811(2003).