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Primary Antibodies

AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody

E11-7007ACite ID: EN0025325DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Q13131
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of Serine 485/491(S-G- SP-V-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody detects endogenous levels of AMPK1/AMPK2 only when phosphorylated at Serine 485 or Serine 491.
ReferencesKim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.
Price225$
Immunohistochemical analysis of paraffin- embedded colon carcinoma, using AMPK1/AMPK2 (Phospho- Ser485/Ser491) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin- embedded colon carcinoma, using AMPK1/AMPK2 (Phospho- Ser485/Ser491) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Western blot analysis using AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody.Line1: The extracts from HeLa cells; Line2: The extracts from HeLa cells treated with heat shock.
Western blot analysis using AMPK1/AMPK2 (Phospho-Ser485/Ser491) Antibody.Line1: The extracts from HeLa cells; Line2: The extracts from HeLa cells treated with heat shock.

Conjugated Antibody

This product is also available conjugated. Format: E11-7007A-Conjugate. Price: $485 / Size: 100μl.

E11-7007A-FITC$485 / 100μlE11-7007A-Biotin$485 / 100μlE11-7007A-HRP$485 / 100μlE11-7007A-AP$485 / 100μlE11-7007A-AF350$485 / 100μlE11-7007A-AF405M$485 / 100μlE11-7007A-AF488$485 / 100μlE11-7007A-AF514$485 / 100μlE11-7007A-AF532$485 / 100μlE11-7007A-AF555$485 / 100μlE11-7007A-AF568$485 / 100μlE11-7007A-AF405S$485 / 100μlE11-7007A-AF405L$485 / 100μlE11-7007A-AF546$485 / 100μlE11-7007A-AF594$485 / 100μlE11-7007A-AF610$485 / 100μlE11-7007A-AF635$485 / 100μlE11-7007A-AF647$485 / 100μlE11-7007A-AF680$485 / 100μlE11-7007A-AF700$485 / 100μlE11-7007A-AF750$485 / 100μlE11-7007A-AF790$485 / 100μlE11-7007A-APC$485 / 100μlE11-7007A-PE$485 / 100μlE11-7007A-Cy3$485 / 100μlE11-7007A-Cy5$485 / 100μlE11-7007A-Cy5.5$485 / 100μlE11-7007A-Cy7$485 / 100μlE11-7007A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.