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Primary Antibodies

Akt (Phospho-Thr308) Antibody

E11-7005ACite ID: EN0025321DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
P31749
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Threonine 308 (M-K-TP-F-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAkt(Phospho-Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Threonine 308.
ReferencesTremblay F, et al. (2005)Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Akt(Phospho-Thr308) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Akt(Phospho-Thr308) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Western blot analysis using Akt(Phospho-Thr308) Antibody: Line1: The extracts from 293 cells untreated; Line2: The extracts from 293 cells treated with insulin.
Western blot analysis using Akt(Phospho-Thr308) Antibody: Line1: The extracts from 293 cells untreated; Line2: The extracts from 293 cells treated with insulin.

Conjugated Antibody

This product is also available conjugated. Format: E11-7005A-Conjugate. Price: $485 / Size: 100μl.

E11-7005A-FITC$485 / 100μlE11-7005A-Biotin$485 / 100μlE11-7005A-HRP$485 / 100μlE11-7005A-AP$485 / 100μlE11-7005A-AF350$485 / 100μlE11-7005A-AF405M$485 / 100μlE11-7005A-AF488$485 / 100μlE11-7005A-AF514$485 / 100μlE11-7005A-AF532$485 / 100μlE11-7005A-AF555$485 / 100μlE11-7005A-AF568$485 / 100μlE11-7005A-AF405S$485 / 100μlE11-7005A-AF405L$485 / 100μlE11-7005A-AF546$485 / 100μlE11-7005A-AF594$485 / 100μlE11-7005A-AF610$485 / 100μlE11-7005A-AF635$485 / 100μlE11-7005A-AF647$485 / 100μlE11-7005A-AF680$485 / 100μlE11-7005A-AF700$485 / 100μlE11-7005A-AF750$485 / 100μlE11-7005A-AF790$485 / 100μlE11-7005A-APC$485 / 100μlE11-7005A-PE$485 / 100μlE11-7005A-Cy3$485 / 100μlE11-7005A-Cy5$485 / 100μlE11-7005A-Cy5.5$485 / 100μlE11-7005A-Cy7$485 / 100μlE11-7005A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.