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Akt (Ab-473) Antibody
Datasheet   

  • Catalog Number:

    E11-7004B

  • Amount:

    100μg/100μl

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    This antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of Serine 473.

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Specificity/Sensitivity:

    Akt (Ab-473) Antibody detects endogenous levels of total Akt protein.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:3000 IHC: 1:50~1:100
    ELISA: 1:10000

  • Swiss-Prot No.:

    P31749

  • Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.

  • Min YH, et al. (2004) Cancer Res; 64(15): 5225-31.
    Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7.

  • Ayala G, et al. (2004) Clin Cancer Res; 10(19): 6572-8.
    Lungu AO, et al. (2004) J Biol Chem; 279(47): 48794-800.

  • Immunohistochemical analysis of paraffin- embedded breast carcinoma tissue, using Akt (Ab-473) Antibody (#B7004). Left: Untreated; Right: Treated with synthesized peptide.

  • Western blot analysis of extracts from HeLa cells treated or untreated with heat shock using Akt (Ab-473) Antibody (#B7004) and Akt (Phospho-Ser473) Antibody (#11168). Line1: Using #B7004; Line2: Using #B7004 preincubated with synthesized peptide.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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