World supplier of antibodies, antigens and kits since 2007
Primary Antibodies

Akt (Phospho-Ser473) Antibody

E11-7004ACite ID: EN0025319DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:100~1:500 IHC: 1:50~1:100
ELISA: 1:5000
P31749
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of Serine473 (Q-F-SP-Y-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAkt(Phospho-Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Serine 473.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin- embedded breast carcinoma, using Akt (Phospho- Ser473) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Immunohistochemical analysis of paraffin- embedded breast carcinoma, using Akt (Phospho- Ser473) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.
Western blot analysis of extracts from HeLa cells treated or untreated with heat shock using Akt(Ab-473) Antibody and Akt (Phospho-Ser473) Antibody.
Western blot analysis of extracts from HeLa cells treated or untreated with heat shock using Akt(Ab-473) Antibody and Akt (Phospho-Ser473) Antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-7004A-Conjugate. Price: $485 / Size: 100μl.

E11-7004A-FITC$485 / 100μlE11-7004A-Biotin$485 / 100μlE11-7004A-HRP$485 / 100μlE11-7004A-AP$485 / 100μlE11-7004A-AF350$485 / 100μlE11-7004A-AF405M$485 / 100μlE11-7004A-AF488$485 / 100μlE11-7004A-AF514$485 / 100μlE11-7004A-AF532$485 / 100μlE11-7004A-AF555$485 / 100μlE11-7004A-AF568$485 / 100μlE11-7004A-AF405S$485 / 100μlE11-7004A-AF405L$485 / 100μlE11-7004A-AF546$485 / 100μlE11-7004A-AF594$485 / 100μlE11-7004A-AF610$485 / 100μlE11-7004A-AF635$485 / 100μlE11-7004A-AF647$485 / 100μlE11-7004A-AF680$485 / 100μlE11-7004A-AF700$485 / 100μlE11-7004A-AF750$485 / 100μlE11-7004A-AF790$485 / 100μlE11-7004A-APC$485 / 100μlE11-7004A-PE$485 / 100μlE11-7004A-Cy3$485 / 100μlE11-7004A-Cy5$485 / 100μlE11-7004A-Cy5.5$485 / 100μlE11-7004A-Cy7$485 / 100μlE11-7004A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.