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Primary Antibodies

4E-BP1 (Ab-45) Antibody

E11-7002BCite ID: EN0025316DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Q13541
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

Altername4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificty Sensitivity

Specificity/Sensitivity4E-BP1 (Ab-45) antibody detects endogenous levels of total 4E-BP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.
ReferencesGingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Price225$
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody.
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (Line1 and 2) and 4E-BP1 (phospho-Thr45) antibody (Line 3 and 4).
Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (Line1 and 2) and 4E-BP1 (phospho-Thr45) antibody (Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E11-7002B-Conjugate. Price: $485 / Size: 100μl.

E11-7002B-FITC$485 / 100μlE11-7002B-Biotin$485 / 100μlE11-7002B-HRP$485 / 100μlE11-7002B-AP$485 / 100μlE11-7002B-AF350$485 / 100μlE11-7002B-AF405M$485 / 100μlE11-7002B-AF488$485 / 100μlE11-7002B-AF514$485 / 100μlE11-7002B-AF532$485 / 100μlE11-7002B-AF555$485 / 100μlE11-7002B-AF568$485 / 100μlE11-7002B-AF405S$485 / 100μlE11-7002B-AF405L$485 / 100μlE11-7002B-AF546$485 / 100μlE11-7002B-AF594$485 / 100μlE11-7002B-AF610$485 / 100μlE11-7002B-AF635$485 / 100μlE11-7002B-AF647$485 / 100μlE11-7002B-AF680$485 / 100μlE11-7002B-AF700$485 / 100μlE11-7002B-AF750$485 / 100μlE11-7002B-AF790$485 / 100μlE11-7002B-APC$485 / 100μlE11-7002B-PE$485 / 100μlE11-7002B-Cy3$485 / 100μlE11-7002B-Cy5$485 / 100μlE11-7002B-Cy5.5$485 / 100μlE11-7002B-Cy7$485 / 100μlE11-7002B-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.