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4E-BP1 (Phospho-Thr45) Antibody
Datasheet   

  • Catalog Number:

    E11-7002A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    Q13541

  • Altername:

    4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I

  • All Sites:

    Human: Thr45 or Thr46; Mouse: Thr44; Rat: Thr44

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    4E-BP1 (phospho-Thr45) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
    ELISA: 1:20000

  • References:

    Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
    Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
    Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
    Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
    Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

  • P-Peptide - +
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (phospho-Thr45) antibody.

  • Western blot analysis of extracts from MDA435 cells untreated or treated with EGF (200nm, 5mins), using 4E-BP1 (Ab-45) antibody (Line1 and 2) and 4E-BP1 (phospho-Thr45) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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4E-BP1 (Phospho-Thr45) Antibody | EnoGene Biotech Co. Ltd