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4E-BP1 (Phospho-Thr36) Antibody
Datasheet   

  • Catalog Number:

    E11-7001A

  • Amount:

    100μg/100μl

  • Swiss-Prot No.:

    Q13541

  • Altername:

    4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I

  • All Sites:

    Human: Thr36 or Thr37; Mouse: Thr35; Rat: Thr35

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    4E-BP1 (phospho-Thr36) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

  • References:

    Gingras AC, et al. Genes Dev Nov. 2005 Feb 15.
    Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64.
    Gingras AC, et al.(1999) Genes Dev:13(11): 1422-37.
    Lal L, et al.(2005) Blood;105(4): 1669-7.

  • P-Peptide - +
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHRL1 (phospho-Ser253) antibody.

  • Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (Line 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (Line 3 and 4).

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg/100μl
  • Price:225$

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