Other Names VIME, vimentin
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Vimentin (Phospho-Ser56) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 56.
ReferencesOta T., Nat. Genet. 36:40-45(2004).
Rasmussen R.K., Electrophoresis 18:588-598(1997).
Gupta A.K., Gene 86:303-304(1990).
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Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells