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Primary Antibodies

Vimentin (Phospho-Ser56) Antibody

E11-1243ACite ID: EN0019365DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
P08670
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names VIME, vimentin
All Sites Human: Ser56
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Vimentin (Phospho-Ser56) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 56.
ReferencesOta T., Nat. Genet. 36:40-45(2004).
Rasmussen R.K., Electrophoresis 18:588-598(1997).
Gupta A.K., Gene 86:303-304(1990).
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Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Phospho- Ser56) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Phospho- Ser56) antibody.
Western blot analysis of extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours), using Vimentin (Phospho-Ser56) antibody.
Western blot analysis of extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours), using Vimentin (Phospho-Ser56) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1243A-Conjugate. Price: $485 / Size: 100μl.

E11-1243A-FITC$485 / 100μlE11-1243A-Biotin$485 / 100μlE11-1243A-HRP$485 / 100μlE11-1243A-AP$485 / 100μlE11-1243A-AF350$485 / 100μlE11-1243A-AF405M$485 / 100μlE11-1243A-AF488$485 / 100μlE11-1243A-AF514$485 / 100μlE11-1243A-AF532$485 / 100μlE11-1243A-AF555$485 / 100μlE11-1243A-AF568$485 / 100μlE11-1243A-AF405S$485 / 100μlE11-1243A-AF405L$485 / 100μlE11-1243A-AF546$485 / 100μlE11-1243A-AF594$485 / 100μlE11-1243A-AF610$485 / 100μlE11-1243A-AF635$485 / 100μlE11-1243A-AF647$485 / 100μlE11-1243A-AF680$485 / 100μlE11-1243A-AF700$485 / 100μlE11-1243A-AF750$485 / 100μlE11-1243A-AF790$485 / 100μlE11-1243A-APC$485 / 100μlE11-1243A-PE$485 / 100μlE11-1243A-Cy3$485 / 100μlE11-1243A-Cy5$485 / 100μlE11-1243A-Cy5.5$485 / 100μlE11-1243A-Cy7$485 / 100μlE11-1243A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.