All Sites Human: Ser83; Mouse: Ser83; Rat: Ser83
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Vimentin (Phospho-Ser83) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 83.
ReferencesFerrari S., Mol. Cell. Biol. 6:3614-3620(1986).
Honore B., Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B., Br. J. Dermatol. 150:252-258(2004).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells