Other Names GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha
All Sites Human: Tyr467/199; Mouse: Tyr467/199; Rat: Tyr467/199
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PI3-kinase p85-subunit α/γ around the phosphorylationsite of tyrosine 467/199 (R-L-YP-E-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody detects endogenous levels of PI3-kinase p85-subunit α/γ only when phosphorylated at tyrosine 467/199.
ReferencesSkolnik E.Y., Cell 65:83-90(1991).
Antonetti D.A., Mol. Cell. Biol. 16:2195-2203(1996).
Van Horn D.J., J. Biol. Chem. 269:29-32(1994).