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Primary Antibodies

PI3-kinasep85-subunitα/γ(Phospho-Tyr467/199) Antibody

E11-1224ACite ID: EN0019109DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
P27986
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names GRB1, P85A, PI3-kinase p85-alpha subunit, PI3K, PI3K p85-alpha, Phosphatidylinositol 3-kinase regulatory alpha subunit, PtdIns-3-kinase p85-alpha
All Sites Human: Tyr467/199; Mouse: Tyr467/199; Rat: Tyr467/199
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PI3-kinase p85-subunit α/γ around the phosphorylationsite of tyrosine 467/199 (R-L-YP-E-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody detects endogenous levels of PI3-kinase p85-subunit α/γ only when phosphorylated at tyrosine 467/199.
ReferencesSkolnik E.Y., Cell 65:83-90(1991).
Antonetti D.A., Mol. Cell. Biol. 16:2195-2203(1996).
Van Horn D.J., J. Biol. Chem. 269:29-32(1994).
Price225$
Western blot analysis of extracts from COS7 cells, treated with H 2 O 2 (100uM, 30mins), using PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody.
Western blot analysis of extracts from COS7 cells, treated with H 2 O 2 (100uM, 30mins), using PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody.
Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody.
Immunofluorescence analysis of NIH/3T3 cells, using PI3-kinase p85-subunit α/γ (Phospho-Tyr467/199) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1224A-Conjugate. Price: $485 / Size: 100μl.

E11-1224A-FITC$485 / 100μlE11-1224A-Biotin$485 / 100μlE11-1224A-HRP$485 / 100μlE11-1224A-AP$485 / 100μlE11-1224A-AF350$485 / 100μlE11-1224A-AF405M$485 / 100μlE11-1224A-AF488$485 / 100μlE11-1224A-AF514$485 / 100μlE11-1224A-AF532$485 / 100μlE11-1224A-AF555$485 / 100μlE11-1224A-AF568$485 / 100μlE11-1224A-AF405S$485 / 100μlE11-1224A-AF405L$485 / 100μlE11-1224A-AF546$485 / 100μlE11-1224A-AF594$485 / 100μlE11-1224A-AF610$485 / 100μlE11-1224A-AF635$485 / 100μlE11-1224A-AF647$485 / 100μlE11-1224A-AF680$485 / 100μlE11-1224A-AF700$485 / 100μlE11-1224A-AF750$485 / 100μlE11-1224A-AF790$485 / 100μlE11-1224A-APC$485 / 100μlE11-1224A-PE$485 / 100μlE11-1224A-Cy3$485 / 100μlE11-1224A-Cy5$485 / 100μlE11-1224A-Cy5.5$485 / 100μlE11-1224A-Cy7$485 / 100μlE11-1224A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.