Other Names KIAA0827, NF-AT5, NFAT5, T cell transcription factor NFAT5, TonE-binding protein, nuclear factor of activated T cells 5, tonicity-responsive enhancer-binding protein
All Sites Human: Ser1197; Mouse: Ser1197
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NFAT5 around the phosphorylation site of serine 1197 (M-L-SP-Q-E).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity NFAT5 (Phospho-Ser1197) antibody detects endogenous levels of NFAT5 only when phosphorylated at serine 1197.
ReferencesJonathan H. Esensten, Nucleic Acids Res., Jul 2005; 33: 3845 - 3854.
Jason Trama, J. Immunol., Nov 2002; 169: 5477.
Cristina López-Rodríguez, PNAS, Feb 2004; 101: 2392.