Other Names CK1-alpha, CKI-alpha, CSNK1A1, Casein kinase I, alpha isoform, EC 2.7.11.1, KC1A, kinase
All Sites Human: Thr321; Mouse: Thr321; Rat: Thr321
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Casein Kinase I α around the phosphorylation site of threonine 321 (A-Q-TP-P-T).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Casein Kinase I α (Phospho-Tyr321) antibody detects endogenous levels of Casein Kinase I α only when phosphorylated at threonine 321.
ReferencesTapia C., FEBS Lett. 349:307-312(1994).
Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
Liu C., l 108:837-847(2002).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells