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Primary Antibodies
CD227/MUC1 (Phospho-Tyr1229) Antibody
Human (Identities = 100%, Positives = 100%)
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227; EMA; Episialin; H23AG; MUC-1; mucin 1; Mucin 1 precursor; mucin 1, transmembrane; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen; Tumor-associated mucin
All Sites Human: Tyr1229
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CD227/MUC1 around the phosphorylation site of tyrosine1229 (S-P-YP-E-K).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CD227/MUC1 (Phospho-Tyr1229) antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.
ReferencesLan M.S., J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J., J. Biol. Chem. 265:15286-15293(1990).
Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J., J. Biol. Chem. 265:15286-15293(1990).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody.
Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using CD227/MUC1 (Phospho-Tyr1229) antibody.
Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-1193A-Conjugate. Price: $485 / Size: 100μl.
E11-1193A-FITC$485 / 100μlE11-1193A-Biotin$485 / 100μlE11-1193A-HRP$485 / 100μlE11-1193A-AP$485 / 100μlE11-1193A-AF350$485 / 100μlE11-1193A-AF405M$485 / 100μlE11-1193A-AF488$485 / 100μlE11-1193A-AF514$485 / 100μlE11-1193A-AF532$485 / 100μlE11-1193A-AF555$485 / 100μlE11-1193A-AF568$485 / 100μlE11-1193A-AF405S$485 / 100μlE11-1193A-AF405L$485 / 100μlE11-1193A-AF546$485 / 100μlE11-1193A-AF594$485 / 100μlE11-1193A-AF610$485 / 100μlE11-1193A-AF635$485 / 100μlE11-1193A-AF647$485 / 100μlE11-1193A-AF680$485 / 100μlE11-1193A-AF700$485 / 100μlE11-1193A-AF750$485 / 100μlE11-1193A-AF790$485 / 100μlE11-1193A-APC$485 / 100μlE11-1193A-PE$485 / 100μlE11-1193A-Cy3$485 / 100μlE11-1193A-Cy5$485 / 100μlE11-1193A-Cy5.5$485 / 100μlE11-1193A-Cy7$485 / 100μlE11-1193A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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