Other Names NF-A1; OCT1; OTF-1; Octamer-binding transcription factor 1; PO21; PO2F1; POU domain, class 2; transcription factor 1; POU2F1
All Sites Human: Ser385; Mouse: Ser388; Rat: Ser274
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human POU2F1/OCT1 around the phosphorylation site of serine385 (R-T-SP-I-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity POU2F1/OCT1 (Phospho-Ser385) antibody detects endogenous levels of POU2F1/OCT1 only when phosphorylated at serine 385.
ReferencesSturm R.A., Genes Dev. 2:1582-1599(1988).
Luchina N.N., Immunol. Lett. 85:237-241(2003).
Gregory S.G., Nature 441:315-321(2006).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells