Other Names 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, P47 phox, p47-phox
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 359 (Q-R-SP-K-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p47 phox (Phospho-Ser359) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 359.
ReferencesVolpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J., Science 245:409-412(1989).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells