Other Names 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, P47 phox, p47-phox
All Sites Human: Ser370; Mouse: Ser370; Rat: Ser369
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 370 (R-P-SP-A-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p47 phox (Phospho-Ser370) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 370.
ReferencesPL Bunger, J. Leukoc. Biol., Jan 2000; 67: 63.
J Rosenthal, Blood, Dec 1996; 88: 4321 - 4329.
Sen-Lin Li, J. Biol. Chem., Jul 1997; 272: 17802.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells