Other Names Early response protein NAK1, GFRP, GFRP1, HMR, N10, N10 nuclear protein, NAK1, NGFI-B, NGFIB, NR4A1, Nuclear hormone receptor NUR/77, Orphan nuclear receptor HMR, TR3 orphan receptor
All Sites Human: Ser351; Mouse: Ser354; Rat: Ser350
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of serine 351 (L-P-SP-K-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Nuclear Receptor NR4A1 (Phospho-Ser351) antibody detects endogenous levels of Nuclear Receptor NR4A1 only when phosphorylated at serine 351.
ReferencesNakai A., Mol. Endocrinol. 4:1438-1443(1990).
Chang C., J. Steroid Biochem. 34:391-395(1989).
Bondy G.P., Cell Growth Differ. 2:203-208(1991).
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Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells