Other Names B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, nucleophosmin
All Sites Human: Thr234; Mouse: Thr232; Rat: Thr232
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 234 (E-K-TP-P-K).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity NPM (Phospho-Thr234) antibody detects endogenous levels of NPM only when phosphorylated at threonine 234.
ReferencesChan W.-Y., Biochemistry 28:1033-1039(1989).
Li X., Biochem. Biophys. Res. Commun. 163:72-78(1989).
Zhang X.T., Biochem. Biophys. Res. Commun. 164:176-184(1989).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells