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Primary Antibodies

NPM (Phospho-Thr234) Antibody

E11-1166ACite ID: EN0018730DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:1000
P06748
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, nucleophosmin
All Sites Human: Thr234; Mouse: Thr232; Rat: Thr232
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 234 (E-K-TP-P-K).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity NPM (Phospho-Thr234) antibody detects endogenous levels of NPM only when phosphorylated at threonine 234.
ReferencesChan W.-Y., Biochemistry 28:1033-1039(1989).
Li X., Biochem. Biophys. Res. Commun. 163:72-78(1989).
Zhang X.T., Biochem. Biophys. Res. Commun. 164:176-184(1989).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours), using NPM (Phospho- Thr234) antibody.
Western blot analysis of extracts from HeLa cells, treated with nocodazole (1ug/ml, 18hours), using NPM (Phospho- Thr234) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1166A-Conjugate. Price: $485 / Size: 100μl.

E11-1166A-FITC$485 / 100μlE11-1166A-Biotin$485 / 100μlE11-1166A-HRP$485 / 100μlE11-1166A-AP$485 / 100μlE11-1166A-AF350$485 / 100μlE11-1166A-AF405M$485 / 100μlE11-1166A-AF488$485 / 100μlE11-1166A-AF514$485 / 100μlE11-1166A-AF532$485 / 100μlE11-1166A-AF555$485 / 100μlE11-1166A-AF568$485 / 100μlE11-1166A-AF405S$485 / 100μlE11-1166A-AF405L$485 / 100μlE11-1166A-AF546$485 / 100μlE11-1166A-AF594$485 / 100μlE11-1166A-AF610$485 / 100μlE11-1166A-AF635$485 / 100μlE11-1166A-AF647$485 / 100μlE11-1166A-AF680$485 / 100μlE11-1166A-AF700$485 / 100μlE11-1166A-AF750$485 / 100μlE11-1166A-AF790$485 / 100μlE11-1166A-APC$485 / 100μlE11-1166A-PE$485 / 100μlE11-1166A-Cy3$485 / 100μlE11-1166A-Cy5$485 / 100μlE11-1166A-Cy5.5$485 / 100μlE11-1166A-Cy7$485 / 100μlE11-1166A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.