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Primary Antibodies

MDM2 (Phospho-Ser186+Ser188) Antibody

E11-1154ACite ID: EN0018683DatasheetCOAMSDS
Human (Identities = 100%, Positives = 100%)
IF: 1:100~1:500 ELISA: 1:20000
Q00987
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Double minute 2 protein, EC 6.3.2.-, Oncoprotein Mdm2, Ubiquitin-protein ligase E3 Mdm2, p53-binding protein Mdm2
All Sites Human: Ser186+Ser188
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MDM2 around the phosphorylation site of serine 186 and serine 188 (H-K-SP-D-SP-I-S).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity MDM2 (Phospho-Ser186+Ser188) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.
ReferencesM Zhou, Blood, Mar 1995; 85: 1608 - 1614.
Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030.
M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) antibody.
Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) antibody.
MDM2 (Phospho-Ser186+Ser188) antibody reacts with epitope-specific phosphopeptide and corresponding non- phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
MDM2 (Phospho-Ser186+Ser188) antibody reacts with epitope-specific phosphopeptide and corresponding non- phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E11-1154A-Conjugate. Price: $485 / Size: 100μl.

E11-1154A-FITC$485 / 100μlE11-1154A-Biotin$485 / 100μlE11-1154A-HRP$485 / 100μlE11-1154A-AP$485 / 100μlE11-1154A-AF350$485 / 100μlE11-1154A-AF405M$485 / 100μlE11-1154A-AF488$485 / 100μlE11-1154A-AF514$485 / 100μlE11-1154A-AF532$485 / 100μlE11-1154A-AF555$485 / 100μlE11-1154A-AF568$485 / 100μlE11-1154A-AF405S$485 / 100μlE11-1154A-AF405L$485 / 100μlE11-1154A-AF546$485 / 100μlE11-1154A-AF594$485 / 100μlE11-1154A-AF610$485 / 100μlE11-1154A-AF635$485 / 100μlE11-1154A-AF647$485 / 100μlE11-1154A-AF680$485 / 100μlE11-1154A-AF700$485 / 100μlE11-1154A-AF750$485 / 100μlE11-1154A-AF790$485 / 100μlE11-1154A-APC$485 / 100μlE11-1154A-PE$485 / 100μlE11-1154A-Cy3$485 / 100μlE11-1154A-Cy5$485 / 100μlE11-1154A-Cy5.5$485 / 100μlE11-1154A-Cy7$485 / 100μlE11-1154A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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MDM2 (Phospho-Ser186+Ser188) Antibody | EnoGene Biotech Co. Ltd