Other Names AFX; AFX1; Afxh; forkhead box protein O4; forkhead domain AFX1; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 (AFX1)
All Sites Human: Thr451; Mouse: Thr452
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FOXO4 around the phosphorylation site of threonine 451 (L-G-TP-P-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity FOXO4 (Phospho-Thr451) antibody detects endogenous levels of FOXO4 only when phosphorylated at threonine 451.
ReferencesPeters U., Hum. Genet. 100:569-572(1997).
Borkhardt A., Oncogene 14:195-202(1997).
Yang Z., J. Biol. Chem. 277:8068-8075(2002).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells