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eNOS (Phospho-Ser615) Antibody
Datasheet   

  • Catalog Number:

    E11-1145A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P29474

  • Other Names:

    Constitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS

  • All Sites:

    Human: Ser615; Mouse: Ser614; Rat: Ser614

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:40000

  • References:

    Janssens S.P., J. Biol. Chem. 267:14519-14522(1992).
    Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
    Marsden P.A., FEBS Lett. 307:287-293(1992).

  • Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

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