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Primary Antibodies

eNOS (Phospho-Ser615) Antibody

E11-1145ACite ID: EN0018634DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:40000
P29474
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Constitutive NOS, EC 1.14.13.39, EC-NOS, ECNOS, Endothelial NOS, NOS, type III, NOS3, NOSIII, Nitric-oxide synthase, endothelial, cNOS
All Sites Human: Ser615; Mouse: Ser614; Rat: Ser614
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of serine 615 (F-N-SP-I-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity eNOS (Phospho-Ser615) antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.
ReferencesJanssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A., FEBS Lett. 307:287-293(1992).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.
Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1145A-Conjugate. Price: $485 / Size: 100μl.

E11-1145A-FITC$485 / 100μlE11-1145A-Biotin$485 / 100μlE11-1145A-HRP$485 / 100μlE11-1145A-AP$485 / 100μlE11-1145A-AF350$485 / 100μlE11-1145A-AF405M$485 / 100μlE11-1145A-AF488$485 / 100μlE11-1145A-AF514$485 / 100μlE11-1145A-AF532$485 / 100μlE11-1145A-AF555$485 / 100μlE11-1145A-AF568$485 / 100μlE11-1145A-AF405S$485 / 100μlE11-1145A-AF405L$485 / 100μlE11-1145A-AF546$485 / 100μlE11-1145A-AF594$485 / 100μlE11-1145A-AF610$485 / 100μlE11-1145A-AF635$485 / 100μlE11-1145A-AF647$485 / 100μlE11-1145A-AF680$485 / 100μlE11-1145A-AF700$485 / 100μlE11-1145A-AF750$485 / 100μlE11-1145A-AF790$485 / 100μlE11-1145A-APC$485 / 100μlE11-1145A-PE$485 / 100μlE11-1145A-Cy3$485 / 100μlE11-1145A-Cy5$485 / 100μlE11-1145A-Cy5.5$485 / 100μlE11-1145A-Cy7$485 / 100μlE11-1145A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.