Other Names C-JUN N-terminal kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 4, EC 2.7.12.2, JNK activating kinase 1, JNK kinase 1, JNKK, JNKK 1, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MKK4, MP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1, c-Jun N- terminal kinase kinase 1, kinase SEK1
All Sites Human: Ser257; Mouse: Ser255; Rat: Ser135
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4/JNKK1 around the phosphorylation site of serine 257 (V-D-SP-I-A).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity SEK1/MKK4/JNKK1 (Phospho-Ser257) antibody detects endogenous levels of SEK1/MKK4/JNKK1 only when phosphorylated at serine 257.
ReferencesBarbara A. Yoshida, Cancer Res., Nov 1999; 59: 5483 - 5487.
S. Diane Yamada, Cancer Res., Nov 2002; 62: 6717 - 6723.
Soula Ganiatsas, PNAS, Jun 1998; 95: 6881.
Jonathan A. Hickson, Cancer Res., Feb 2006; 66: 2264 - 2270.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells