Other Names ATP-regulated potassium channel ROM-K, ATP-sensitive inward rectifier potassium channel 1, IRK1, KAB-1, KCNJ1, Kir1.1, Potassium channel, inwardly rectifying, subfamily J, member 1, ROMK1
All Sites Human: Ser44; Mouse: Ser25; Rat: Ser44
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ROMK/Kir1.1 around the phosphorylation site of serine 44/25 (L-V-SP-K-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ROMK/Kir1.1 (Phospho-Ser44/25) antibody detects endogenous levels of ROMK/Kir1.1 only when phosphorylated at serine 44/25.
ReferencesDW Lundgren, Proc. Soc. Exp. Biol. Med., Oct 1997; 216: 57.
Dana Yoo, J. Biol. Chem., Jun 2003; 278: 23066 - 23075.
W. S. Lee, Am J Physiol Renal Physiol, Jun 1995; 268: 1124.