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ROMK/Kir1.1(Phospho-Ser44/25)Antibody
Datasheet   

  • Catalog Number:

    E11-1121A
  • Amount:

    100μg
  • Swiss-Prot No.:

    P48048
  • Other Names:

    ATP-regulated potassium channel ROM-K, ATP-sensitive inward rectifier potassium channel 1, IRK1, KAB-1, KCNJ1, Kir1.1, Potassium channel, inwardly rectifying, subfamily J, member 1, ROMK1
  • All Sites:

    Human: Ser44; Mouse: Ser25; Rat: Ser44
  • Storage/Stability:

    Store at -20℃/1 year
  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human ROMK/Kir1.1 around the phosphorylation site of serine 44/25 (L-V-SP-K-D).
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Specificity/Sensitivity:

    ROMK/Kir1.1 (Phospho-Ser44/25) antibody detects endogenous levels of ROMK/Kir1.1 only when phosphorylated at serine 44/25.
  • Reactivity:

    Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 90%, Positives = 100%)
  • Applications:

    IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
  • References:

    DW Lundgren, Proc. Soc. Exp. Biol. Med., Oct 1997; 216: 57.
    Dana Yoo, J. Biol. Chem., Jun 2003; 278: 23066 - 23075.
    W. S. Lee, Am J Physiol Renal Physiol, Jun 1995; 268: 1124.
  • P-peptide - +
  • P-peptide - +
    Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.
    Immunofluorescence analysis of A549 cells, using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.
  • ROMK/Kir1.1 (Phospho-Ser44/25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
  • Price:

    225$

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

AbPromise