Other Names C3G, CRK SH3-binding GNRP, GRF2, RPGF1, Rap guanine nucleotide exchange factor 1, guanine nucleotide-releasing factor 2
All Sites Human: Tyr504; Mouse: Tyr514
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RapGEF1 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity RapGEF1 (Phospho-Tyr504) antibody detects endogenous levels of RapGEF1 only when phosphorylated at tyrosine 504.
ReferencesTanaka S., Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994).
Knudsen B., J. Biol. Chem. 269:32781-32787(1994).
Matsuda M., J. Biol. Chem. 271:14468-14472(1996).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells