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Primary Antibodies

RapGEF1 (Phospho-Tyr504) Antibody

E11-1120ACite ID: EN0018489DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 ELISA: 1:1000
Q13905
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names C3G, CRK SH3-binding GNRP, GRF2, RPGF1, Rap guanine nucleotide exchange factor 1, guanine nucleotide-releasing factor 2
All Sites Human: Tyr504; Mouse: Tyr514
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RapGEF1 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity RapGEF1 (Phospho-Tyr504) antibody detects endogenous levels of RapGEF1 only when phosphorylated at tyrosine 504.
ReferencesTanaka S., Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994).
Knudsen B., J. Biol. Chem. 269:32781-32787(1994).
Matsuda M., J. Biol. Chem. 271:14468-14472(1996).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using RapGEF1 (Phospho-Tyr504) antibody.
Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using RapGEF1 (Phospho-Tyr504) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1120A-Conjugate. Price: $485 / Size: 100μl.

E11-1120A-FITC$485 / 100μlE11-1120A-Biotin$485 / 100μlE11-1120A-HRP$485 / 100μlE11-1120A-AP$485 / 100μlE11-1120A-AF350$485 / 100μlE11-1120A-AF405M$485 / 100μlE11-1120A-AF488$485 / 100μlE11-1120A-AF514$485 / 100μlE11-1120A-AF532$485 / 100μlE11-1120A-AF555$485 / 100μlE11-1120A-AF568$485 / 100μlE11-1120A-AF405S$485 / 100μlE11-1120A-AF405L$485 / 100μlE11-1120A-AF546$485 / 100μlE11-1120A-AF594$485 / 100μlE11-1120A-AF610$485 / 100μlE11-1120A-AF635$485 / 100μlE11-1120A-AF647$485 / 100μlE11-1120A-AF680$485 / 100μlE11-1120A-AF700$485 / 100μlE11-1120A-AF750$485 / 100μlE11-1120A-AF790$485 / 100μlE11-1120A-APC$485 / 100μlE11-1120A-PE$485 / 100μlE11-1120A-Cy3$485 / 100μlE11-1120A-Cy5$485 / 100μlE11-1120A-Cy5.5$485 / 100μlE11-1120A-Cy7$485 / 100μlE11-1120A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.