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RapGEF1 (Phospho-Tyr504) Antibody
Datasheet   

  • Catalog Number:

    E11-1120A

  • Amount:

    100μg

  • Swiss-Prot No.:

    Q13905

  • Other Names:

    C3G, CRK SH3-binding GNRP, GRF2, RPGF1, Rap guanine nucleotide exchange factor 1, guanine nucleotide-releasing factor 2

  • All Sites:

    Human: Tyr504; Mouse: Tyr514

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human RapGEF1 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P).

  • Purification:

    The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    RapGEF1 (Phospho-Tyr504) antibody detects endogenous levels of RapGEF1 only when phosphorylated at tyrosine 504.

  • Reactivity:

    Human, Mouse

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:1000

  • References:

    Tanaka S., Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994).
    Knudsen B., J. Biol. Chem. 269:32781-32787(1994).
    Matsuda M., J. Biol. Chem. 271:14468-14472(1996).

  • Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using RapGEF1 (Phospho-Tyr504) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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