Other Names DNA repair protein RAD51 homolog 1, RAD51A, RECA
All Sites Human: Tyr315; Mouse: Tyr315
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RAD51 around the phosphorylation site of tyrosine 315 (K-I-YP-D-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity RAD51 (Phospho-Tyr315) antibody detects endogenous levels of RAD51 only when phosphorylated at tyrosine 315.
ReferencesShinohara A., Nat. Genet. 4:239-243(1993).
Yoshimura Y., Nucleic Acids Res. 21:1665-1665(1993).
Schmutte C., Cancer Res. 59:4564-4569(1999).
R.E., Daly M., Cancer Epidemiol. Biomarkers Prev. 10:955-960(2001).