Other Names MKI67IP, NIFK, Nucleolar protein NIFK, RNA binding protein
All Sites Human: Thr234; Mouse: Thr257
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NIFK around the phosphorylation site of threonine 234 (G-P-TP-P-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity NIFK (Phospho-Thr234) antibody detects endogenous levels of NIFK only when phosphorylated at threonine 234.
ReferencesJ G Li, J. Bacteriol., Oct 1990; 172: 5884 - 5891.
Masatoshi Takagi, J. Biol. Chem., Jun 2001; 276: 25386 - 25391.
AM Hirsch, Mol. Biol. Evol., Jan 1995; 12: 16 - 27.
J G Li, J. Bacteriol., Oct 1990; 172: 5884 - 5891.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells