Other Names HsMAD1, MAD1, MAD1-like 1, MD11, MD1L1, Mitotic arrest deficient-like protein 1, Mitotic checkpoint MAD1 protein-homolog, Mitotic spindle assembly checkpoint protein MAD1, TXBP181, Tax binding protein-181, Hmad1
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MAD1 around the phosphorylation site of serine 428 (E-Y-SP-P-Q).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MAD1 (Phospho-Ser428) antibody detects endogenous levels of MAD1 only when phosphorylated at serine 428.
ReferencesStefanie Gehring, J. Biol. Chem., Mar 2000; 275: 10413.
Brian J. Tunquist, J. Cell Biol., Dec 2003; 163: 1231.
Yoichi Iwanaga, J. Biol. Chem., Aug 2002; 277: 31005 - 31013.