Other Names LEF1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
All Sites Human: Ser42; Mouse: Ser40; Rat: Ser40
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LEF-1 around the phosphorylation site of serine 42 (E-I-SP-H-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity LEF-1 (Phospho-Ser42) antibody detects endogenous levels of LEF-1 only when phosphorylated at serine 42.
ReferencesWaterman M.L., Genes Dev. 5:656-669(1991).
Hovanes K., Nucleic Acids Res. 28:1994-2003(2000).
Kobielak A., Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells