Other Names ACO1, Aconitate hydratase, EC 4.2.1.3, Ferritin repressor protein, IRE-BP 1, IRE1, IREBP1, IRP1, aconitase, citrate hydro-lyase, iron regulatory protein 1, iron-responsive element binding protein 1
All Sites Human: Ser138; Mouse: Ser138; Rat: Ser138
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IREB1 around the phosphorylation site of serine 138 (A-D-SP-L-Q).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity IREB1 (Phospho-Ser138) antibody detects endogenous levels of IREB1 only when phosphorylated at serine 138.
ReferencesHirling H., Nucleic Acids Res. 20:33-39(1992).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Rouault T.A., Proc. Natl. Acad. Sci. U.S.A. 87:7958-7962(1990).