Other Names CD25 antigen, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain precursor, TAC antigen, p55
All Sites Human: Ser268; Mouse: Ser264
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IL-2Rα/CD25 around the phosphorylation site of serine 268 (R-K-SP-R-R).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity IL-2Rα/CD25 (Phospho-Ser268) antibody detects endogenous levels of IL-2Rα/CD25 only when phosphorylated at serine 268.
ReferencesNikaido T., Nature 311:631-635(1984).
Bamborough P., Structure 2:839-851(1994).
Stauber D.J., Proc. Natl. Acad. Sci. U.S.A. 103:2788-2793(2006).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells