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Primary Antibodies
IκB-α (Phospho-Tyr305) Antibody
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1
All Sites Human: Tyr305; Mouse: Tyr302; Rat: Tyr302
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of tyrosine 305 (L-P-YP-D-D).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity IκB-α (Phospho-Tyr305) antibody detects endogenous levels of IκB-α only when phosphorylated at tyrosine 305.
ReferencesHaskill S., Cell 65:1281-1289(1991).
Jungnickel B., J. Exp. Med. 191:395-402(2000).
Ganchi P.A., Mol. Biol. Cell 3:1339-1352(1992).
Jungnickel B., J. Exp. Med. 191:395-402(2000).
Ganchi P.A., Mol. Biol. Cell 3:1339-1352(1992).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using IκB-α (Phospho-Tyr305) antibody.
Western blot analysis of extracts from COS7 cells, treated with nocodazole (1ug/ml, 16hours), using IκB-α (Phospho-Tyr305) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-1053A-Conjugate. Price: $485 / Size: 100μl.
E11-1053A-FITC$485 / 100μlE11-1053A-Biotin$485 / 100μlE11-1053A-HRP$485 / 100μlE11-1053A-AP$485 / 100μlE11-1053A-AF350$485 / 100μlE11-1053A-AF405M$485 / 100μlE11-1053A-AF488$485 / 100μlE11-1053A-AF514$485 / 100μlE11-1053A-AF532$485 / 100μlE11-1053A-AF555$485 / 100μlE11-1053A-AF568$485 / 100μlE11-1053A-AF405S$485 / 100μlE11-1053A-AF405L$485 / 100μlE11-1053A-AF546$485 / 100μlE11-1053A-AF594$485 / 100μlE11-1053A-AF610$485 / 100μlE11-1053A-AF635$485 / 100μlE11-1053A-AF647$485 / 100μlE11-1053A-AF680$485 / 100μlE11-1053A-AF700$485 / 100μlE11-1053A-AF750$485 / 100μlE11-1053A-AF790$485 / 100μlE11-1053A-APC$485 / 100μlE11-1053A-PE$485 / 100μlE11-1053A-Cy3$485 / 100μlE11-1053A-Cy5$485 / 100μlE11-1053A-Cy5.5$485 / 100μlE11-1053A-Cy7$485 / 100μlE11-1053A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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