Other Names ARE element RNA-binding protein AUF1, AU-rich element RNA-binding protein 1, AUF1, HNRPD, Heterogeneous nuclear ribonucleoprotein D, Heterogeneous nuclear ribonucleoprotein D0, ROD
All Sites Human: Ser52; Mouse: Ser52
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human hnRPD around the phosphorylation site of serine 83 (N-S-SP-P-R).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity hnRPD (Phospho-Ser83) antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.
ReferencesKajita Y., J. Biol. Chem. 270:22167-22175(1995).
Dempsey L.A., Genomics 49:378-384(1998).
Tolnay M., Biochem. J. 338:417-425(1999).
Grosset C., Cell 103:29-40(2000).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells