Other Names HMG14, High-mobility group nucleosome binding domain 1, Nonhistone chromosomal protein HMG-14
All Sites Human: Ser21; Mouse: Ser20; Rat: Ser21
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HMG14 around the phosphorylation site of serine 21 (R-R-SP-A-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity HMG14 (Phospho-Ser21) antibody detects endogenous levels of HMG14 only when phosphorylated at serine 21.
ReferencesMihael H. Polymeropoulos. Nucleic Acids Res., Jul 1991; 19: 3753.
LJ Arwood. J. Biol. Chem., Jun 1990; 265: 9771 - 9777.
Thomas Hildmann. Genome Res., Apr 1999; 9: 360 - 372.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells