Other Names CASP-1, EC 3.4.22.36, ICE, IL-1 beta converting enzyme, IL-1BC, IL1BCE, caspase-1, interleukin-1 beta convertase, interleukin-1 beta converting enzyme, p45
All Sites Human: Ser376; Mouse: Ser374
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Caspase 1 around the phosphorylation site of serine 376 (R-F-SP-F-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Caspase 1 (Phospho-Ser376) antibody detects endogenous levels of Caspase 1 only when phosphorylated at serine 376.
ReferencesThornberry N.A., Nature 356:768-774(1992).
Feng Q., Genomics 84:587-591(2004).
Agostini L., Immunity 20:319-325(2004).
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells