Other Names Metavinculin, VCL, VINC
All Sites Human: Tyr821; Mouse: Tyr821; Rat: Tyr822
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Vinculin around the phosphorylation site of tyrosine 821 (S-G-YP-R-I).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Vinculin (Phospho-Tyr821) antibody detects endogenous levels of Vinculin only when phosphorylated at tyrosine 821.
ReferencesTricia A. Holwell, J. Lipid Res., Aug 1999; 40: 1440.
M Galou, J. Cell Biol., May 1996; 133: 853.
PV Hornbeck, J. Immunol., Oct 1993; 151: 4013.
M Ishizaki, Invest. Ophthalmol. Vis. Sci., Nov 1993; 34: 3320.