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Primary Antibodies

Vinculin (Phospho-Tyr821) Antibody

E11-1017ACite ID: EN0017903DatasheetCOAMSDS
Human, Mouse, Rat
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
Q64727
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Metavinculin, VCL, VINC
All Sites Human: Tyr821; Mouse: Tyr821; Rat: Tyr822
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Vinculin around the phosphorylation site of tyrosine 821 (S-G-YP-R-I).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Vinculin (Phospho-Tyr821) antibody detects endogenous levels of Vinculin only when phosphorylated at tyrosine 821.
ReferencesTricia A. Holwell, J. Lipid Res., Aug 1999; 40: 1440.
M Galou, J. Cell Biol., May 1996; 133: 853.
PV Hornbeck, J. Immunol., Oct 1993; 151: 4013.
M Ishizaki, Invest. Ophthalmol. Vis. Sci., Nov 1993; 34: 3320.
Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vinculin (Phospho-Tyr821) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vinculin (Phospho-Tyr821) antibody.
Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) antibody.
Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-1017A-Conjugate. Price: $485 / Size: 100μl.

E11-1017A-FITC$485 / 100μlE11-1017A-Biotin$485 / 100μlE11-1017A-HRP$485 / 100μlE11-1017A-AP$485 / 100μlE11-1017A-AF350$485 / 100μlE11-1017A-AF405M$485 / 100μlE11-1017A-AF488$485 / 100μlE11-1017A-AF514$485 / 100μlE11-1017A-AF532$485 / 100μlE11-1017A-AF555$485 / 100μlE11-1017A-AF568$485 / 100μlE11-1017A-AF405S$485 / 100μlE11-1017A-AF405L$485 / 100μlE11-1017A-AF546$485 / 100μlE11-1017A-AF594$485 / 100μlE11-1017A-AF610$485 / 100μlE11-1017A-AF635$485 / 100μlE11-1017A-AF647$485 / 100μlE11-1017A-AF680$485 / 100μlE11-1017A-AF700$485 / 100μlE11-1017A-AF750$485 / 100μlE11-1017A-AF790$485 / 100μlE11-1017A-APC$485 / 100μlE11-1017A-PE$485 / 100μlE11-1017A-Cy3$485 / 100μlE11-1017A-Cy5$485 / 100μlE11-1017A-Cy5.5$485 / 100μlE11-1017A-Cy7$485 / 100μlE11-1017A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.