Other Names EC 1.14.16.4, TPH, TRPH, tryptophan 5-hydroxylase 1, tryptophan 5-monooxygenase 1
All Sites Human: Ser260; Mouse: Ser260; Rat: Ser263
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Tryptophan Hydroxylase around the phosphorylation site of serine 260 (H-S-SP-D-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Tryptophan Hydroxylase (Phospho-Ser260) antibody detects endogenous levels of Tryptophan Hydroxylase only when phosphorylated at serine 260.
ReferencesBoulalard S., Nucleic Acids Res. 18:4257-4257(1990).
Tipper J.P., Arch. Biochem. Biophys. 315:445-453(1994).
Wang G.A., J. Neurochem. 71:1769-1772(1998).