Other Names JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
All Sites Human: Ser208; Mouse: Ser208; Rat: Ser208
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 208 (N-L-SP-P-N).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Smad3 (Phospho-Ser208) antibody detects endogenous levels of Smad3 only when phosphorylated at serine 208.
ReferencesWen Zhang. J. Biol. Chem., Dec 2000; 275: 39237.
Brendan D. Looyenga. Mol. Endocrinol., Jul 2007; 10.1210/me.2006-0402.
Jian Xu. J Androl, Mar 2003; 24: 192.